TY - JOUR
T1 - Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis
AU - Graham, Robert I.
AU - Morin, Benoit
AU - Lapointe, Renée
AU - Nealis, Vincent G.
AU - Lucarotti, Christopher J.
PY - 2008/9
Y1 - 2008/9
N2 - A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis. The complete nucleotide sequences of genomic segments S2-S5 and S7-S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5' (AGUUU......UUUGUGC) 3' were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae. Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana.
AB - A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis. The complete nucleotide sequences of genomic segments S2-S5 and S7-S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5' (AGUUU......UUUGUGC) 3' were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae. Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana.
U2 - 10.1007/s00705-008-0175-x
DO - 10.1007/s00705-008-0175-x
M3 - Journal Article
SN - 0304-8608
VL - 153
SP - 1759
EP - 1763
JO - Archives of Virology
JF - Archives of Virology
IS - 9
ER -